Valorization of Shrimp Shell Waste into Functional Chitosan: Recent Advances, Analytical Challenges, and Future Perspectives

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Review Article

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Vol. 2 No. 1 (2026): In Progress

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Bishir, S., Ramkat, R. C., Makatiani, J. K., & Pili, N. N. (2026). Valorization of Shrimp Shell Waste into Functional Chitosan: Recent Advances, Analytical Challenges, and Future Perspectives. Global Waste Valorization, 2(1), 36-55. https://doi.org/10.65773/gwv.2.1.143

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Authors

Sadiq Bishir*
Department of Biological Sciences, School of Sciences and Aerospace Studies, Moi University, Eldoret, Kenya ; Department of Biological Sciences, Umaru Musa Yar’adua University, Nigeria
Rose Chepchirchir Ramkat
Department of Biological Sciences, School of Sciences and Aerospace Studies, Moi University, Eldoret, Kenya ; Africa Center of Excellence II in Phytochemicals Textiles and Renewable Energy (ACEII-PTRE), Moi University, Eldoret, Kenya
Jacqueline Kubochi Makatiani
Department of Biological Sciences, School of Sciences and Aerospace Studies, Moi University, Eldoret, Kenya ; Africa Center of Excellence II in Phytochemicals Textiles and Renewable Energy (ACEII-PTRE), Moi University, Eldoret, Kenya
Njira Njira Pili
Department of Biological Sciences, School of Sciences and Aerospace Studies, Moi University, Eldoret, Kenya

Abstract

The increasing accumulation of shrimp shell waste from seafood processing presents both an environmental challenge and an opportunity for sustainable biomass valorization within the circular bioeconomy. Chitosan, a biodegradable and biocompatible polysaccharide obtained through chitin deacetylation, has emerged as a versatile biomaterial with broad applications in biomedicine, agriculture, environmental remediation, food packaging, and advanced functional materials. This review critically evaluates recent advances in the production of chitosan from shrimp shell waste, emphasizing extraction technologies, quality determinants, structural characterization, and analytical evaluation. Conventional chemical extraction is critically compared with emerging green approaches, including microwave-, ultrasonic-, autoclave-, enzymatic-, fermentation-, and deep eutectic solvent-assisted processes, highlighting their effects on yield, degree of deacetylation, molecular weight, scalability, and environmental sustainability. Current characterization and analytical methods are comparatively assessed, with particular emphasis on the complementary role of 3,5-dinitrosalicylic acid (DNSA)-based assays for reducing-end quantification during chitosan depolymerization. The review further discusses advances in functionalized chitosan derivatives, nanocomposites, industrial translation, and sustainable production strategies while identifying standardization of extraction and analytical protocols as a critical requirement for improving reproducibility and facilitating commercial-scale production. Collectively, this review provides an integrated perspective on current progress, remaining challenges, and future research priorities for developing high-quality chitosan from shrimp shell waste.

Keywords:
chitosan, shrimp shell waste, structural characterization, DNSA assay, circular bioeconomy

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References

[1]Gao, M., Tang, H., & Zhu, H. (2024). Advances in extraction, utilization, and development of chitin/chitosan and its derivatives from shrimp shell waste. Comprehensive Reviews in Food Science and Food Safety, 23, e70008. https://doi.org/10.1111/1541-4337.70008

[2]Hosney, A., Ullah, S., & Barčauskaitė, K. (2022). A Review of the Chemical Extraction of Chitosan from Shrimp Wastes and Prediction of Factors Affecting Chitosan Yield by Using an Artificial Neural Network. Marine Drugs, 20(11), 675. https://doi.org/10.3390/md20110675

[3]Ali, S. A., et al. (2024). Enhancing physical characteristics and antibacterial efficacy of chitosan through investigation of microwave-assisted chemically formulated chitosan-coated ZnO and chitosan/ZnO physical composite. Scientific Reports, 14(1), 9348. https://doi.org/10.1038/s41598-024-58862-6

[4]Hosney, A., Urbonavičius, M., Varnagiris, Š., Ignatjev, I., Ullah, S., & Barčauskaitė, K. (2025). Feasibility study on optimizing chitosan extraction and characterization from shrimp biowaste via acidic demineralization. Biomass Conversion and Biorefinery, 15(8), 12673–12687. https://doi.org/10.1007/s13399-024-06017-y

[5]Zulkarnain, Z., Mukti, W. A. H., & Kurniawan, K. (2024). Sustainable and environment-friendly management of shrimp processing waste through high-quality chitosan production. Leuser Journal of Environmental Studies, 2(2), 95–100. https://doi.org/10.60084/ljes.v2i2.229

[6]Rinaudo, M. (2006). Chitin and chitosan: Properties and applications. Progress in Polymer Science, 31(7), 603–632. https://doi.org/10.1016/j.progpolymsci.2006.06.001

[7]Weißpflog, J., et al. (2021). Characterization of chitosan with different degree of deacetylation and equal viscosity in dissolved and solid state – Insights by various complimentary methods. International Journal of Biological Macromolecules, 171, 242–261. https://doi.org/10.1016/j.ijbiomac.2021.01.010

[8]Chen, S., et al. (2023). Preparation and application of chitosan-based medical electrospun nanofibers. International Journal of Biological Macromolecules, 226, 410–422. https://doi.org/10.1016/j.ijbiomac.2022.12.056

[9]Mojumdar, A., Upadhyay, A. K., Raina, V., & Ray, L. (2019). A simple and rapid colorimetric method for the estimation of chitosan produced by microbial degradation of chitin waste. Journal of Microbiological Methods, 158, 66–70. https://doi.org/10.1016/j.mimet.2019.02.001

[10]Liyanage, C. S., Gonapinuwala, S. T., Fernando, C. A. N., & De Croos, M. D. S. T. (2022). Physico-chemical properties of chitosan extracted from Whiteleg shrimp (Litopenaeus vannamei) processing shell waste in Sri Lanka. Sri Lanka Journal of Aquatic Sciences, 27(2), 107–122. https://doi.org/10.4038/sljas.v27i2.7600

[11]Olaosebikan, A. O., Kehinde, O. A., Tolulase, O. A., & Victor, E. B. (2021). Extraction and characterization of chitin and chitosan from Callinectes amnicola and Penaeus notialis shell wastes. Journal of Chemical Engineering and Materials Science, 12(1), 1–30. https://doi.org/10.5897/JCEMS2020.0353

[12]Darzi Arbabi, H., Motamedzadegan, A., Pirdashti, M., Shahrokhi, B., & Arzideh, S. M. (2022). Extraction and physicochemical characterization of chitosan from Litopenaeus vannamei shells. Iranian Journal of Chemistry and Chemical Engineering, Online First. https://doi.org/10.30492/ijcce.2022.527512.4659

[13]El Knidri, H., El Khalfaouy, R., Laajeb, A., Addaou, A., & Lahsini, A. (2016). Eco-friendly extraction and characterization of chitin and chitosan from the shrimp shell waste via microwave irradiation. Process Safety and Environmental Protection, 104, 395–405. https://doi.org/10.1016/j.psep.2016.09.020

[14]Putra, N. R., Rizkiyah, D. N., Che Yunus, M. A., & Qomariyah, L. (2024). Towards a greener future: Bioactive compounds extraction from shrimp shells using eco‐friendly techniques. eFood, 5(3), e146. https://doi.org/10.1002/efd2.146

[15]Chonanant, C., et al. (2024). Biocomposite scaffolds based on chitosan extraction from shrimp shell waste for cartilage tissue engineering application. ACS Omega, acsomega.4c02910. https://doi.org/10.1021/acsomega.4c02910

[16]Saravanan, A., Parthasarathy, V., & Kumar, P. S. (2024). Antimicrobial, antifungal, and antioxidant activities of the extracted chitosan from shrimp shell waste and its bioadsorption study of heavy metals from the aqueous solution. Biomass Conversion and Biorefinery, 14(15), 18109–18119. https://doi.org/10.1007/s13399-023-04078-z

[17]Çelebi, M., Tav, A., Kaya, M. A., & Özdemir, Z. Ö. (2026). Scalable production of low-molecular-weight chitosan: Comparative study of conventional, microwave, and autoclave-assisted methods. Polymers, 18(2), 213. https://doi.org/10.3390/polym18020213

[18]Hosney, A., et al. (2026). Optimizing chitosan extraction and characterization from shrimp shells: Deproteinization and exploratory machine learning-based similarity model. ChemEngineering, 10(5), 56. https://doi.org/10.3390/chemengineering10050056

[19]Younes, I., & Rinaudo, M. (2015). Chitin and chitosan preparation from marine sources. Structure, properties and applications. Marine Drugs, 13(3), 1133–1174. https://doi.org/10.3390/md13031133

[20]Apriyanti, D. T., Susanto, H., & Rokhati, N. (2018). Influence of microwave irradiation on extraction of chitosan from shrimp shell waste. Reaktor, 18(1), 45. https://doi.org/10.14710/reaktor.18.1.45-50

[21]Andoko, A., Prasetya, R., Cakra, B. D., Syah, M. Z., & Santoso, A. (2025). Autoclave-assisted multi-objective optimization of the deacetylation process in chitosan production from shrimp shell waste via response surface methodology. Results in Engineering, 27, 106353. https://doi.org/10.1016/j.rineng.2025.106353

[22]Rahman, M. M., & Maniruzzaman, M. (2023). A new route of production of the meso-porous chitosan with well-organized honeycomb surface microstructure from shrimp waste without destroying the original structure of native shells: Extraction, modification and characterization study. Results in Engineering, 19, 101362. https://doi.org/10.1016/j.rineng.2023.101362

[23]El-Araby, A., Janati, W., Ullah, R., Ercisli, S., & Errachidi, F. (2024). Chitosan, chitosan derivatives, and chitosan-based nanocomposites: Eco-friendly materials for advanced applications (a review). Frontiers in Chemistry, 11, 1327426. https://doi.org/10.3389/fchem.2023.1327426

[24]Kumirska, J., et al. (2010). Application of spectroscopic methods for structural analysis of chitin and chitosan. Marine Drugs, 8(5), 1567–1636. https://doi.org/10.3390/md8051567

[25]Lavertu, M., et al. (2003). A validated ¹H NMR method for the determination of the degree of deacetylation of chitosan. Journal of Pharmaceutical and Biomedical Analysis, 32(6), 1149–1158. https://doi.org/10.1016/S0731-7085(03)00155-9

[26]Kasaai, M. R. (2010). Determination of the degree of N-acetylation for chitin and chitosan by various NMR spectroscopy techniques: A review. Carbohydrate Polymers, 79(4), 801–810. https://doi.org/10.1016/j.carbpol.2009.10.051

[27]Brugnerotto, J., Lizardi, J., Goycoolea, F. M., Argüelles-Monal, W., Desbrières, J., & Rinaudo, M. (2001). An infrared investigation in relation with chitin and chitosan characterization. Polymer, 42(8), 3569–3580. https://doi.org/10.1016/S0032-3861(00)00713-8

[28]Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31(3), 426–428. https://doi.org/10.1021/ac60147a030

[29]Gusakov, A. V., Kondratyeva, E. G., & Sinitsyn, A. P. (2011). Comparison of two methods for assaying reducing sugars in the determination of carbohydrase activities. International Journal of Analytical Chemistry, 2011, 1–4. https://doi.org/10.1155/2011/283658.

[30]Badawy, M. E. I. (2012). A new rapid and sensitive spectrophotometric method for determination of a biopolymer chitosan. International Journal of Carbohydrate Chemistry, 2012(1), 139328. https://doi.org/10.1155/2012/139328

[31]Sali, S., Thampi, M., & Jisha, M. S. (2026). Chitosan-based nanocomposites: A sustainable frontier in plant growth regulation and postharvest preservation. Physiological and Molecular Plant Pathology, 141, 103026. https://doi.org/10.1016/j.pmpp.2025.103026

[32]Wang, J., Han, W., Wang, W., Wang, L., Zou, J., & Wei, D. (2026). Chitosan-based food preservation films: A review of structural modification, functional properties, and preservation applications. Reactive and Functional Polymers, 227, 106847. https://doi.org/10.1016/j.reactfunctpolym.2026.106847

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